RNA sequencing is a powerful method for qualitative and quantitative analysis of the transcriptome and regulatory RNAs. Transcriptomics is used for differential gene expression analysis, detection of alternative splicing and characterisation of rare or novel transcripts.
Procomcure Biotech offers comprehensive RNA Sequencing Services for different scientific purposes.
| Sample | ≥20 µl, ≥25 ng/µl High or Low quality RNA | |
| Library preparation | QuantSeq 3’ mRNA-Seq Library Prep Kit (Lexogen) | |
Inclusions:
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GEx analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports); free data transfer of FastQ, Bam files and Reports | |
| Sequencing metrics | Performed on Illumina NextSeq/NovaSeq with 1x≥50 bp single-end run; Seq throughput: 10/20 M SE read per sample; Q30 ≥ 85% | |
| Quality control | Full quality assurance and guaranteed specifications | |
| Turnaround time | min. 2 – max. 6 weeks | |
| Prices | Download our pricelist below for current prices | |
| Sample | ≥20 µl, ≥50 ng/µl High quality RNA (RIN>7) | |
| Library preparation | Nextflex Rapid Directional RNA-Seq kit 2.0 Kit with Poly(A) Beads 2.0 (PerkinElmer) | |
Inclusions:
| mRNA-Seq analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports) ; free data transfer of FastQ, Bam files and Reports | |
| Sequencing metrics | Performed on Illumina NextSeq/NovaSeq with 2x≥150 bp paired-end run; Seq throughput: 50/100 M PE read per sample; Q30 ≥ 80% | |
| Quality control | Full quality assurance and guaranteed specifications | |
| Turnaround time | min. 2 – max. 6 weeks | |
| Prices | Download our pricelist below for current prices | |
| Sample | ≥20 µl, ≥50 ng/µl High or Low quality RNA | |
| Library preparation | Nextflex Rapid Directional RNA-Seq kit 2.0 Kit with RiboNaut rRNA Depletion HMR (PerkinElmer) | |
Inclusions:
| RNA-Seq analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports) ; free data transfer of FastQ, Bam files and Reports | |
| Sequencing metrics | Performed on Illumina NextSeq/NovaSeq with 2x≥150 bp paired-end run; Seq throughput: 100/200 M PE read per sample; Q30 ≥ 80% | |
| Quality control | Full quality assurance and guaranteed specifications | |
| Turnaround time | min. 2 – max. 6 weeks | |
| Prices | Download our pricelist below for current prices | |
| Sample | ≥20 µl, ≥50 ng/µl High quality RNA | |
| Library preparation | Nextflex Small RNA-Seq Kit v3 (PerkinElmer) | |
Inclusions:
| miRNA-Seq analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports); free data transfer of FastQ, Bam files and Reports | |
| Sequencing metrics | Performed on Illumina NextSeq/NovaSeq with 2x≥40 bp paired-end run; Seq throughput: 20/40 M PE read per sample; Q30 ≥ 85% | |
| Quality control | Full quality assurance and guaranteed specifications | |
| Turnaround time | min. 2 – max. 6 weeks | |
| Prices | Download our pricelist below for current prices | |