NGS SERVICES: RNA ANALYSIS

Our RNA services in Next Generation Sequencing…

RNA Sequencing

RNA sequencing is a powerful method for qualitative and quantitative analysis of the transcriptome and regulatory RNAs. Transcriptomics is used for differential gene expression analysis, detection of alternative splicing and characterisation of rare or novel transcripts.

Procomcure Biotech offers comprehensive RNA Sequencing Services for different  scientific purposes.

  • Gene Expression Profing (GEx): determines the gene-level abundance and differential expression of coding transcripts.
  • mRNA-Seq : targeting poly(A) RNAs provides a sensitive option for detection and quantitation of even rare coding transcript variants.
  • RNA-Seq : RNA samples are enriched for both mRNA and non-coding RNAs to provide a comprehensive view of the whole transcriptome.
  • Small RNA-Seq : targets small regulatory RNAs such as miRNA for profiling and discovery.

GEx Specifications

Sample ≥20 µl, ≥25 ng/µl High or Low quality RNA
Library preparation QuantSeq 3’ mRNA-Seq Library Prep Kit (Lexogen)
Inclusions:
  • Sequencing
  • Bioinformatics
  • Raw data transfer
GEx analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports); free data transfer of FastQ, Bam files and Reports
Sequencing metrics Performed on Illumina NextSeq/NovaSeq with 1x≥50 bp single-end run; Seq throughput: 10/20 M SE read per sample; Q30 ≥ 85%
Quality control Full quality assurance and guaranteed specifications
Turnaround time min. 2 – max. 6 weeks
Prices Download our pricelist below for current prices  
Price List

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mRNA-Seq Specifications

Sample ≥20 µl, ≥50 ng/µl High quality RNA (RIN>7)
Library preparation Nextflex Rapid Directional RNA-Seq kit 2.0 Kit with Poly(A) Beads 2.0 (PerkinElmer)
Inclusions:
  • Sequencing
  • Bioinformatics
  • Raw data transfer
mRNA-Seq analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports) ; free data transfer of FastQ, Bam files and Reports
Sequencing metrics Performed on Illumina NextSeq/NovaSeq with 2x≥75 bp paired-end run; Seq throughput: 50/100 M PE read per sample; Q30 ≥ 80%
Quality control Full quality assurance and guaranteed specifications
Turnaround time min. 2 – max. 6 weeks
Prices Download our pricelist below for current prices  
Price List

Send download link to:

Above you will receive a link to our price list. With downloading, you agree that your e-mail address will be stored for statistical and marketing purposes, and to possibly being contacted by us. We will not sign you up to a newsletter. Our Terms and Conditions and Privacy Policy.

RNA-Seq Specifications

Sample ≥20 µl, ≥50 ng/µl High or Low quality RNA
Library preparation Nextflex Rapid Directional RNA-Seq kit 2.0 Kit with RiboNaut rRNA Depletion HMR (PerkinElmer)
Inclusions:
  • Sequencing
  • Bioinformatics
  • Raw data transfer
RNA-Seq analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports) ; free data transfer of FastQ, Bam files and Reports
Sequencing metrics Performed on Illumina NextSeq/NovaSeq with 2x≥75 bp paired-end run; Seq throughput: 100/200 M PE read per sample; Q30 ≥ 80%
Quality control Full quality assurance and guaranteed specifications
Turnaround time min. 2 – max. 6 weeks
Prices Download our pricelist below for current prices  
Price List

Send download link to:

Above you will receive a link to our price list. With downloading, you agree that your e-mail address will be stored for statistical and marketing purposes, and to possibly being contacted by us. We will not sign you up to a newsletter. Our Terms and Conditions and Privacy Policy.

Small RNA-Seq Specifications

Sample

≥20 µl, ≥50 ng/µl High quality RNA

Library preparation

Nextflex Small RNA-Seq Kit v3 (PerkinElmer) 

Inclusions:

  • Sequencing
  • Bioinformatics
  • Raw data transfer

Small RNA-Seq analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, mapping, annotation, differential expression and clinical reports) ; free data transfer of FastQ, Bam files and Reports

Sequencing metrics

Performed on Illumina NextSeq/NovaSeq with 2x≥40 bp paired-end run; Seq throughput: 20/40 M PE read per sample; Q30 ≥ 85% 

Quality controlFull quality assurance and guaranteed specifications
Turnaround timemin. 2 – max. 6 weeks
PricesDownload our pricelist below for current prices 
Price List

Send download link to:

Above you will receive a link to our price list. With downloading, you agree that your e-mail address will be stored for statistical and marketing purposes, and to possibly being contacted by us. We will not sign you up to a newsletter. Our Terms and Conditions and Privacy Policy.

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