Whole genome sequencing (WGS) provides the most comprehensive analysis of genome variance and structure. WGS enables accurate detection of single nucleotide and structural variations of human genomes.
Whole genome bisulfite sequencing (WGBS) reveals differentially methylated sites, which can be visualized on the genome at single-nucleotide resolution.
Procomcure Biotech offers WGS/WGBS services for accurate variant detection using the Illumina NovaSeq 6000 and Dragen Bio-IT platforms.
| Sample | ≥20 µl, ≥50 ng/µl High quality DNA | |
| Library preparation | PerkinElmer/NEB/Zymo | |
Inclusions:
| WGS analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis* (filtering, trimming, mapping, annotation and variant reports); free data transfer of FastQ, Bam files and Reports | |
| Sequencing metrics | Performed on Illumina NovaSeq 6000 with 2×150 bp paired-end run; Seq throughput: 30x coverage, based on genome size (human: 90 Gbp read per sample); Q30 ≥ 80% | |
| Quality control | Full quality assurance and guaranteed specifications | |
| Turnaround time | min. 2 – max. 6 weeks | |
| Prices | Download our pricelist below for current prices. | |
| *optional content | ||
Whole exome sequencing (WES) is a powerful tool for investigation of genetic variance across the protein coding regions of the human genome. WES provides high confidence in variant calls for the identification of inherited rare Mendelian disorders, complex diseases and somatic mutations in cancer.
Procomcure Biotech offers Human WES service for accurate variant detection using the Illumina NovaSeq 6000 and Dragen Bio-IT platforms.
| Sample | ≥20 µl, ≥5 ng/µl High quality DNA | |
| Library preparation | IDT | |
Inclusions:
| WES analysis includes sample quality control, automated library preparation, final QC, sequencing and full bioinformatic analysis* (filtering, trimming, mapping, annotation and variant reports); free data transfer of FastQ, Bam files and Reports | |
| Sequencing metrics | Performed on Illumina NovaSeq 6000 with 2×150 bp paired-end run; Seq throughput: custom or 100x /200x coverage, (12/18 Gb read per sample); Q30 ≥ 80 | |
| Quality control | Full quality assurance and guaranteed specifications | |
| Turnaround time | min. 2 – max. 6 weeks | |
| Prices | Download our pricelist below for current prices. | |
| *optional content | ||
| Standard Shotgun Metagenome Analysis (20M PE reads) | ||
| Comprehensive Shotgun Metagenome Analysis (40M PE reads) | ||
| Deep Shotgun Metagenome Analysis (60M PE reads) | ||
| Somatic Hotspot Panel Seq – KRAS, NRAS, BRAF, EGFR, TP53 Mutational Hotspot Analysis from FFPE | ||
| Targeted Sequencing (Target-Seq) – Gene Panels (please specify your demand) | ||
| Long Read Amplicon Sequencing (0.2 M , 2×300 bp PE reads or please specify) | ||
| Download our price list below for current prices | ||