Procomcure’s microbiome NGS (Next-Generation Sequencing) service is a process of analyzing the genetic material of microorganisms present in a given biological sample, such as soil, water, gut, or skin, using high-throughput DNA sequencing technology.
The microbiome NGS service provides a comprehensive analysis of the microbial community composition, diversity, and function present in a particular environment, which can help in understanding the role of microbes in various biological processes.
|Sample||≥20 µl, ≥5 ng/µl High quality DNA|
KAPA HiFi HotStart Ready Mix Kit (Roche) and Nextera XT v2 Index Kit (Illumina)
Microbial diversity analysis includes sample quality control, library preparation, final QC, sequencing and full bioinformatic analysis (filtering, trimming, and metagenomic report*); free data transfer of FastQ files and Reports
Performed on Illumina MiSeq/NextSeq 2000 with 2×300 bp paired-end run; Seq throughput: 0.2M PE read per sample; Q30 ≥ 70%
|Quality control||Full quality assurance and guaranteed specifications|
|Turnaround time||min. 2 – max. 6 weeks|